Purification and characterization of a thaumatin-like protein-1 with polyphenol oxidase activity found in Prunus mume
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منابع مشابه
Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.)
Polyphenol oxidase from apricot (Prunus armeniaca) (PaPPO) was purified in its latent form (L-PaPPO), and the molecular weight was determined to be 63 kDa by SDS-PAGE. L-PaPPO was activated in the presence of substrate at low pH. The activity was enhanced by CuSO4 and low concentrations (≤ 2 mM) of SDS. PaPPO has its pH and temperature optimum at pH 4.5 and 45 °C for catechol as substrate. It s...
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In fruits and vegetables, browning occurs due to the oxidation of polyphenols. Oxidative browning is catalysed by polyphenol oxidase (PPO; monophenol, dihydroxyphenyl alanine:oxygen oxidoreductase; EC.1.14.18.1). When fresh products are damaged oxidative browning occurs, and this is an economic problem for producers and consumers. Polyphenoloxidase, which is a copper-containing enzyme, catalyse...
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Polyphenol oxidase (PPO) was purified from fresh ginseng roots using acetone precipitation, carboxymethyl (CM)-Sepharose chromatography, and phenyl-Sepharose chromatography. Two isoenzymes (PPO 1 and PPO 2) were separated using an ion-exchange column with CM-Sepharose. PPO 1 was purified up to 13.2-fold with a 22.6% yield. PPO 2 bound to CM-Sepharose, eluted with NaCl, and was purified up to 22...
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Prunus mume (mei), which was domesticated in China more than 3,000 years ago as ornamental plant and fruit, is one of the first genomes among Prunus subfamilies of Rosaceae been sequenced. Here, we assemble a 280M genome by combining 101-fold next-generation sequencing and optical mapping data. We further anchor 83.9% of scaffolds to eight chromosomes with genetic map constructed by restriction...
متن کاملPartial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...
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ژورنال
عنوان ژورنال: RSC Advances
سال: 2020
ISSN: 2046-2069
DOI: 10.1039/d0ra05659f